【摘要】 目的 探讨成人急性白血病 (AL)免疫分型标准及其意义 ,了解成人AL抗原表达规律和免疫亚型分布情况。方法 细胞膜抗原采用单克隆抗体三色直接免疫荧光标记法 ,胞浆内抗原采用双色直标法用流式细胞术测定。分型标准试用抗体积分系统。结果 (1)未分化型2.3%;单纯型54.0%,其中急性髓系白血病 (AML)43.6%,淋系10.3%;变异型37.9 %,其中髓系28.7%,淋系9.1%;混合型5.7 %。(2)AL患者CD34、CD38HLA -DR均阳性约70 %,M3 患者HLA -DR与CD34均阴性 ,CD38强阳性 ;AML变异型伴CD7、CD19阳性最常见 ,其次为CD2、CD4、CD56 ;B -ALL变异型依次为CD33、CD13阳性。CD117对髓系特异性强。结论 本方法直接准确地反映成人AL免疫分型特点。更多还原

【Abstract】 Objective To explore the criteria and significance of immunological classification of adult acute leukemia. Methods Immunofluorescence labeled with three and two color monoclonal antibodies directly were used to analyze the surface antigen and cytoplasmic antigen. Cytoplasmic antigen was detected by flow cytometry additionally. An antibody integral system was developed for the immunologic classification. Results Specimens from 87 patients with adult acute leukemia were detected. 1) The undifferentiated phenotype account for 2.3%, simple phenotype 54.0%(Acute myeloid Leukemia 43.6%,acute lymphoid leukemia 10.3%); variant phenotype 37.9%(myeloid lineage 28.7%,lymphoid lineage 9.1%), and mixed phenotype 5.7%. 2) All CD34,CD38 and HLA-DR expressed positively in 70%of AL patients. HLA-DR and CD34 were negative, but CD38 was strongly positive in M3 patients. CD7, CD19, CD2, CD4, CD56 expressed positively in variant AML and decreased in turn. CD33 and CD13 expressed positively in variant B-All. The expression of CD117 was specific in myeloid leukemia. Conclusion The criteria can reflect the characteristic of Immunophenotype of adult acute leukemia directly and exactly.更多还原