【摘要】 在碱性介质中,谷氨酸对硫氰化钾 鲁米诺化学发光体系有增敏作用,建立了用硫氰化钾 鲁米诺 氨基酸体系测定谷氨酸的新方法。该法灵敏度高,操作简单、快速。用该方法测定谷氨酸的线性范围为0.01～1.0μg·ml-1,检出限可达0.01μg·ml-1,采样频率为190次·h-1,对5μg·ml-1的谷氨酸连续平行测量10次,RSD为1.2%。用该方法对皮革屑中酶法提取的谷氨酸含量进行了测定,并与氨基酸测定仪测定的结果进行了比较,结果满意。更多还原

【Abstract】 It was found that in an alkaline medium of pH 10.5, the chemiluminescence emitted by the reaction between potassium thiocyanate and luminol is sensitized by the presence of glutamic acid, and that the magnitude of enhanced sensitivity keeps a linear relationship with the concentration of glutamic acid in the range of 0.01～1.0μg·ml^-1. Based on these facts and in combination with the flow-injection technique, a new FI-chemiluminescence method for the determination of glutamic acid is proposed, and the FI-manifold is also designed and fabricated. By this installation, glutamic acid in leather scraps is determined rapidly and simply, giving a detection limit of 0.01μg·ml^-1 and sampling frequency of 190 sample per hour. RSD′s found by 10 successive determination of glutamic acid at the concentration level of 5μg·ml^-1 is 1.2%. The results obtained by this installation checked very well with those obtained by the amino-acid determinator.更多还原