【摘要】 使用四溴荧光素(TBFS)作为蛋白质的染色剂,建立了一种测定蛋白质的分子吸收光谱分析新体系———BSA 四溴荧光素,体系十分简单,BSA浓度在0.11～60.0μg·ml-1范围符合比耳定律;测定15.0μg·ml-1BSA溶液10次,求得相对标准偏差为1.26%,桑德尔灵敏度为0 094μg·cm-2。可直接用于血清样品中蛋白质的测定,测得质量控制血清样品中蛋白质质量为35.4±2.4mg,与标准值36.9mg吻合。回收率在97.0%～108.3%之间,结果满意。更多还原

【Abstract】 In a citric acid-Na₂HPO₄ buffer solution of pH 2.2, tetrabromofluorescein （TBFS） combines with BSA to give a compound having its absorption maxima at 530nm which is 16nm longer than the absorption maxima of TBFS itself. Based on this new and simple reaction system, TBFS is used as a spectrometric probe in the molecular absorption spectrometric determination of protein. Beer′s law is obeyed in the range of 0.11～60.0μg of BSA per ml of solution. Precision test at the concentration level of 15.0μg·ml^-1 of BSA, the RSD′s （n=10） found is 1.26%. The Sandell′s sensitivity of this reaction is 0.094μg·cm^-2. In its application to the direct determination of protein in a control blood serum sample, a results of 35.4±2.4mg is obtained which agrees very well with the standard value （36.9mg） of this control sample. Recoveries found were in the range of 97.0%～108.3%.更多还原