【摘要】 [目的 ]观察不同浓度的番茄红素 (lycopene ,Ly)或与维生素E(VitaminE)合用对苯并 (a)芘 (benzo(a)pyrene ,BaP)诱导BALB/c 3T3细胞转化的影响。 [方法 ]每组 14瓶 ,每瓶接种 10 4个细胞 ,2 4h后加受试物及苯并 (a)芘 ,3d后 2瓶用结晶紫方法测定细胞毒性 ,其余 12瓶换含DMEM/F12转化表达培养液。每周换液 2次 ,2 5d后 10瓶固定 ,计数直径>2mm转化灶 ;其余 2瓶用定量的双抗体夹心ELISA方法测定细胞中p5 3蛋白的含量。 [结果 ]各种受试物及其混合物的细胞相对存活率均 >90 %。 0 .5、1、5 μmol/L的番茄红素可以阻止苯并 (a)芘诱导的细胞转化作用 ,细胞中p5 3蛋白的含量处在较低水平。然而当番茄红素浓度升高至 10 μmol/L时 ,番茄红素反而降低了抗转化的效果 ,细胞中p5 3蛋白的含量增加。维生素E存在能增强番茄红素抗苯并 (a)芘导致转化作用。 [结论 ]番茄红素阻止苯并 (a)芘诱导的细胞转化作用与其抗氧化作用方式相似 ,未发现番茄红素有类似 β 胡萝卜素的辅致癌作用 ;维生素E能增强番茄红素抗转化效果。更多还原

【Abstract】 To investigate the effect of lycopene alone or in combination with vitamin E on BALB/c-3T3 cell transformation induced by benzo(a)pyrene. BALB/c-3T3 cells were plated at a density of 10,000 cells per plate in fourteen replicates/each treatment. After 24 hours,the test compounds(lycopene,lycopene and vitamin E,BaP) were added in medium,and the control cells received the vehicle and BaP,the cells were cultured for 3 days. Two plates from treated group were used for determination of the cytotoxicity,and other twelve plates were incubated in fresh DMEM/F12.The medium was changed twice a week,for 25 days of culture. Ten plates were fixed and foci in a diameter of more than 2 mm were counted;the remaining two plates were used for determination of p53 protein by sandwich ELISA. Tested chemicals lycopene and lycopene with vitamin E showed non-toxic or slightly toxic in cytotoxicity test. Transformation frequency induced by BaP was reduced by addition of 0.5 μmol/L,1 μmol/L,5 μmol/L lycopene,and the content of p53 protein in cells was very low. However,a higher dose of 10 μmol/L lycopene reduced this anti-transformed ability and p53 protein accumulated. Vitamin E enhanced the effect of lycopene on reduction of transformation frequency induced by BaP. [Conclusion] The effective manner of lycopene of reduction in the transformation frequency induced by BaP is similar to its antioxidation. Lycopene has not been found the co-carcinogenic effect as β-carotene. Vitamin E may enhance the preventive effect of lycopene.更多还原