【摘要】 [目的]研究不同剂量UVA照射对体外培养B₁6小鼠黑素瘤细胞的形态、增殖率、黑素含量和酪氨酸酶活性的影响。[方法]选用B₁6小鼠黑素瘤细胞 ,经不同剂量的UVA照射后 ,采用MTT法测定细胞的增殖 ,参照HideyaAndo等的方法测定酪氨酸酶活性和黑素含量。[结果]UVA照射后 ,黑素含量增加 （P<0.01） ,酪氨酸酶活性提高 （P<0.05） ,细胞增殖率上升 ,照射剂量在0.6、1.2、1.8J/cm2时 ,细胞增殖率的变化有显著性 （t=2.495、2.645、3.081,均为P<0.05）。[结论]随着照射剂量的增加 ,黑素含量增加 ,酪氨酸酶活性提高 ,细胞的增殖率增加 ,但UVA的促黑素瘤细胞增殖作用是有限的更多还原

【Abstract】 Objective To investigate the effects of different doses of ultraviolet A(UVA)irradiation on the morphology,prolifˉeration,melanogenesis,tyrosinase activity of the cultured B16murine melanoma cells.[Methods] After B16melanoma cells were irradiated with UVA,MTTspectrometry was used to determine the proliferation of cells,Hideya Ando technique was applied to measure the synthesis of melanin,and the tyrosinase activity.[Results]It was found that a marked increase in melanin content(P<0.01)and tyrosinase activity(P<0.05)after irradiation with UVA.UVA could increase melanoma cell proliferation.[Conclusion]UVA could increase B16murine melanoma cell melanogenesis,tyrosinase activity and induce the proliferation in a dose-dependent manner.Howˉever,the effect of UVA irradiation on the induction of melanocyte proliferation was limited.更多还原