节点文献

PCNA、P16、MMP9在人参皂甙Rg3抗肝癌淋巴道转移中的表达及意义

Expression and Significance of PCNA, P16 and MMP 9 in the Lymphatic Metastasis Models with Ginsenoside Rg3

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 刘基巍赵翌富力吕申李莹燕秋

【Author】 LIU Ji-wei, ZHAO Yi, FU Li,et al. Department of Oncology, The First Affiliated Hospital of Dalian Medical University,Dalian 116011

【机构】 大连医科大学附属第一医院肿瘤科大连富生天然药物开发研究所大连医科大学病理教研室大连医科大学生化教研室 116011辽宁大连116011辽宁大连116011116027

【摘要】 目的 :通过检测PCNA、P16及MMP 9在人参皂甙Rg3抗荷瘤小鼠淋巴道转移中的表达 ,探讨人参皂甙Rg3抗肿瘤作用的机制。方法 :建立小鼠肝癌淋巴道转移模型 ,应用免疫组织化学方法检测PCNA、P16及MMP 9在各实验组 (Rg3预防组、Rg3治疗组、Rg3+DDP合用组、DDP阳性对照组和生理盐水阴性对照组 )的原发瘤及相应转移瘤 (淋巴结 )中的表达水平。结果 :应用人参皂甙Rg3组较未用药组 ,PCNA及MMP 9在原发瘤的表达减少 ,P16的表达增加 ,差异显著 (P <0 0 0 1) ;而在转移瘤中的变化不明显。结论 :人参皂甙Rg3抗淋巴道转移作用的机制与上调P16表达及下调PCNA和MMP9表达有关。

【Abstract】 Objective:To detect the expression of PCNA, P16 and MMP 9 in tumor with lymphatic metastasis in mice treated with Ginsenoside Rg3, and to explore the mechanism involved in this process.Methods:To establish the transplanted tumor lymphatic metastasis models which were categoried into 5 groups, including Ginsenoside Rg3 pretreatment, treatment,combination-treatment, positive and negative control groups. Immunohistochemical assay was used to detect the expression of the PCNA, P16 and MMP 9 in the primary and metastatic tumors.Results:The expression of P16 was significantly increased, whereas those of PCNA and MMP9 decreased compared with that of saline group (P<0.001).Conclusion:The anti-Lymphatic metastasis mechanisms of Ginsenoside Rg3 are related to the up-regulation of p16 expression and down-regulation of PCNA and MMP 9 expression.

【关键词】 人参皂甙Rg3淋巴道转移P16PCNAMMP9
【Key words】 Ginsenoside Rg3Lymphatic MetastasisP16PCNAMMP 9
  • 【文献出处】 临床肿瘤学杂志 ,Chinese Clinical Oncology , 编辑部邮箱 ,2004年03期
  • 【分类号】R735.7
  • 【被引频次】68
  • 【下载频次】436
节点文献中: 

本文链接的文献网络图示:

本文的引文网络