【摘要】 目的 :探讨近距离小剂量放疗对血管平滑肌细胞 (VSMC)纤维黏连蛋白 (FN)和弹性蛋白基因表达的影响及其机制。方法 :以 7、14、2 8Gy60 Co射线辐射培养的大鼠主动脉VSMC弹性蛋白及FNmRNA表达的影响 ,无辐射为对照组。结果 :7Gy放疗组对VSMC弹性蛋白及FNmRNA表达与对照组比较差异无统计学意义 ,而 14、2 8Gy放疗组FNmRNA分别为0 .72± 0 .12和 0 .4 9± 0 .0 9,与对照组 (0 .95± 0 .0 7)比较差异有统计学意义 (P <0 .0 5 )。 14和 2 8Gy辐射后细胞弹性蛋白mRNA水平分别为1.93± 0 .2 5及 2 .16± 0 .2 2 ,与对照组 (1.0 7± 0 .11)比较差异有统计学意义 (P <0 .0 5 )。结论 :14、2 8Gy60 Co射线辐射可以抑制FNmRNA及加强弹性蛋白mRNA的表达。更多还原

【Abstract】 Objective:To study the effects of radiotherapy on the gene expession of fibronectin (FN) and elastin in the cultured rat vascular smooth muscle cells (VSMCs). Method: Rat VSMCs were cultured in DMEM containing 10% FBS and were radiated by 60Co at a doses of 0,7,14,28 Gy respectively. The mRNA level of fibronectin and elastin were studied by reveries-transcriptative competitive PCR(RT PCR) . Result:The elastin mRNA levels were increased from 1.07± 0.11 to 1.93± 0.25 and 2.16± 0.22 in VSMC with 14,28 Gy radiation respectively (P< 0.05). The FN mRNA levels were decreased from 0.95± 0.07 to 0.72± 0.12 and 0.49± 0.09 in VSMC with 14,28 Gy radiation respectively(P< 0.05). Conclusion:radiotheapy inhabits the gene expression of FN and increased the gene expression of elastin in VSMC.更多还原