【摘要】 目的　原位检测幽门螺杆菌 (Hp)感染时胃粘膜活检标本中肿瘤坏死因子 α(TNF α)和白细胞介素 1β(IL 1β)的产生及Fas抗原的表达 ,并分析它们之间的关系。 方法　采集 13例Hp阴性受检者和 3 2例Hp阳性受检者的胃粘膜活检标本 ,通过放免法、免疫组化法观察并比较两组胃粘膜活检标本中TNF α和IL 1β的产生及Fas抗原的表达。 结果　胃粘膜活检标本中TNF α和IL 1β的分泌在Hp阳性组显著高于Hp阴性组 (P <0 .0 1) ,但与胃粘膜的病理分型无显著相关性 (P >0 .0 5 ) ;Hp阳性组的胃粘膜组织切片中TNF α、IL 1β和Fas抗原表达显著高于Hp阴性组 ;Hp阳性组中TNF α和IL 1β产生与胃粘膜Fas抗原的表达呈显著正相关 (P <0 .0 5 )。 结论　通过原位检测发现Hp感染可以使局部细胞因子TNF α和IL 1β产生增加 ,造成局部的炎症微环境 ,从而上调Fas抗原表达 ,导致胃粘膜损伤更多还原

【Abstract】 Objective To examine the production in situ of tumor necrosis factor-alpha(TNF-α) and interleukin-1 beta(IL-1β) in Hp-associated gastritis,and find the correlation between the cytokines and Fas antigen.Methods Biopsy samples were collected from 13 Hp negative subjects and 32 Hp positive subjects.TNF-α,IL-1β and Fas antigen were detected by radioimmunoassay(RIA),immunohistochemical method.The correlation of them has also been studied.Results TNF-α and IL-1β in Hp positive group were significantly higher than those in Hp negative group(P<0.05).However,it seemed no significant correlation between concentrations of TNF-α,IL-1β and the histological type of gastritis(P> 0.05).The levels of TNF-α,IL-1β and the expression of Fas antigen in Hp positive subjects were significantly higher than those in Hp negative subjects(P<0.05).We also found a highly correlation between the production of TNF-α,IL-1β and Fas antigen expression(P<0.05).Conclusions Hp infection induces the production of TNF-α and IL-1β,and forms the local inflammatory microenvironment,which can stimulate the higher expression of Fas antigen.更多还原