【摘要】 目的 :观察脱细胞真皮基质 (ADM )全层皮肤缺损创面移植后的组织学变化 ,了解ADM的生物学特点。方法 :在Wistar大鼠背部制作全层皮肤缺损创面 ,移植异体ADM ;分别于术后 1、2、3、4周大体观察ADM表面情况 ,取标本作HE染色 ,光镜观察 ,并进行血管计数检查。结果 :成纤维细胞伴随血管自创底逐渐向ADM内生长 ,于术后 2周血管浸润ADM全层。整个观察过程均无明显炎细胞浸润 ,ADM中胶原网架结构仍然保留。结论 :ADM支持成纤维细胞浸润、血管再生 ,血管化过程约在 2周内完成 ,异体移植后亦不引起免疫排斥反应。更多还原

【Abstract】 Objective: To investigate the biological properties of acellular dermal matrix (ADM) as an allograft.Method:Two layers of allogenic ADM were implanted to full-thickness skin defects made on the dorsa of rats.Respectively,the overlying layer of ADM was removed at 1 week,2 week,3 week,4 week after implanting and the deeper ADM was observed macroscopically.Then, samples were taken for histological observation on ADM,and the number of blood vessels of ADM were counted. Result: 1 week after implanting, the surface of the deeper ADM was florid , and newly developed blood vessels had penetrated two-thirds of ADM.2weeks after implanting, extensively spotty microvascular bleeding was found, and blood vessels had extended to the uppermost region of ADM.3 weeks and 4 weeks after implanting,the overlying layer of ADM gradually dried to a crust, and obvious bleeding from the deeper ADM was observed,and the number of blood vessels did not increase significantly(P>0.05).It was also found fibroblasts accompanying newly developed vessels infiltrated ADM ,but no obvious inflammatory cells infiltration at all times.Moreover, the collagen meshwork of ADM retained its original organized structure.Conclusion: ADM could support fibroblasts infiltration, neovascularization and could be completely vascularized within 2 weeks after transplanting .Also, ADM induced no immune rejection.更多还原