【摘要】 目的 :探讨天然胡萝卜素对口腔颊黏膜癌前病变逆转中的局部免疫调节作用。方法 :用二甲基苯并蒽 (DMBA)诱发制作地鼠口腔颊黏膜癌前病变模型。将 65只地鼠分为 4组 ,空白对照组 5只 ,3个实验组各 2 0只。对口腔黏膜癌前病变地鼠以 0 .5 %天然胡萝卜素溶液灌胃 ( 15mg/kg/d ,5d/周× 4周 )或在口腔颊黏膜癌前病变区贴敷天然胡萝卜素口腔膜 ( 1cm× 1cm /10mg ,5d/周× 4周 )。应用流式细胞仪检测空白对照组、癌前病变组和灌胃组、贴膜组治疗 4周和停药后 6周肿瘤坏死因子 (TNF -α)和白细胞介素 10 (IL -10 )表达量。结果 :地鼠颊黏膜癌前病变组TNF -α和IL -10表达量明显高于空白对照组。经用天然胡萝卜素治疗后 ,灌胃组和局部贴膜组均显示TNF -α表达量明显下降 (P <0 .0 1) ;贴膜组可使IL -10表达量降低 (P >0 .0 5 ) ,而灌胃用药组IL -10表达量无明显降低 ,与癌前病变组比较无明显差异 (P >0 .0 5 ) .结论 :天然胡萝卜素对地鼠颊黏膜癌前病变具有一定的局部免疫调节作用 ,其确切的作用机制有待深入研究。更多还原

【Abstract】 Objective:Probe into the local immunoregulation effect of natural carotene on dimethyI-benzanthracene(DMBA)-induced oral buccal macosa premalignant lesion in golden hamsters.Method: Oral buccal macosa premalignant lesion in golden hamsters were induced by DMBA. The hamsters were divided into 4groups, 20 animals in each, except for the natural control group which had 5. Oral premalignant lesion were treated by oral administration of 0.5% natural carotene (15mg·kg-1·d-1, 5d/w, for 4 weeks) and topical application of membrane of natural carotene(1cm×1cm/10mg, 5d/w, for 4 weeks). Expression levels of IL-10 and TNF-α were detected using flowcytometry before and after treating.Result:Expression levels was much higher in oral premalignant lesion group than that in healthy control group. TNF-α expression levels both oral administration and topical application of natual carotene were obviously descend after treating(P<0.01); IL-10 were descend in topical application group(P<0.05),but there was no significant difference from between the oral administration and the oral premalignant lesion group(P>05).Conclusion: Natural carotene showed the local immunoregulation effect on oral buccal macosa premalignant lesion in golden hamsters.But mechanism of action needs to be further studied.更多还原