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地钱总黄酮提取的研究
STUDY ON LEACHING AND PURIFYING METHOD OF FLAVONES FROM MARCHANTIA POLYMORPHA L.
【摘要】 用正交试验法对地钱(MarchantiapolymorphaL.)中的黄酮类化合物提取工艺进行了优选研究,采用正交表L16(45),以提取时间、提取温度、提取次数、提取剂中乙醇含量以及料液比为因素,以总黄酮显色液的吸光度为指标进行试验。得到了最佳提取参数及结果:温度为70℃,时间0.5h,提取次数3次,提取溶剂为80%的乙醇,料液比为1∶15,提取率1.90%,纯度95.83%。
【Abstract】 The optimum technology of extracting flavones in Marchantia polymorpha L.was studied by orthogonal test.An orthogonal test table L16(45) was used with the factors of extracting time,temperature,number of extraction times,ethanol concentration and ratio of solid-to-liquid.The content of flavones was determined by spectrophotometry.The optimum technology was:temperature 70 ℃,time 0.5 h,three times of extraction,80% EtOH as extracting solvent,stock ratio 1∶15.The extraction yield is 1.90%,and its purification about 95.83% with this method.
- 【文献出处】 林产化学与工业 ,Chemistry & Industry of Forest Products , 编辑部邮箱 ,2004年02期
- 【分类号】TQ461
- 【被引频次】15
- 【下载频次】184