【摘要】 目的 :探讨血管紧张素转换酶 (ACE)基因多态性及血管紧张素Ⅱ (AⅡ )活性在阻塞性睡眠呼吸暂停低通气综合征 (OSAHS)患者高血压发病中的作用。方法 :应用聚合酶链反应 ,对 30例OSAHS并发高血压患者 (OSAHS并发高血压组 ) ,30例血压正常的OSAHS患者 (OSAHS血压正常组 )ACE基因插入 /缺失 (I/D)多态性和等位基因的分布频率进行检测。以 30例健康体检者为正常对照组。同时测定其血清AⅡ含量。结果 :OSAHS并发高血压组与OSAHS血压正常组及正常对照组比较 ,ACE基因II基因型明显增高 (P <0 .0 5和P <0 .0 1) ,I等位基因出现频率也明显增高 (均P <0 .0 1) ;OSAHS血压正常组与正常对照组比较 ,ACE基因多态性及I等位基因出现频率差异无统计学意义 (均P >0 .0 5 )。OSAHS并发高血压组及OSAHS血压正常组血清AⅡ含量分别为 (10 9.83± 2 3.72 )和 (10 9.5 0± 2 7.6 5 )ng/L ,其差异无统计学意义 (P >0 .0 5 ) ;分别与正常对照组 [(90 .5 7± 16 .35 )ng/L]比较 ,差异有统计学意义 (均P <0 .0 1)。结论 :ACE基因I等位基因和II基因型频率增加是OSAHS并发高血压发病的重要危险因素 ,说明遗传因素是影响OSAHS发生高血压的重要因素之一。OSAHS患者血清AⅡ含量明显增高 ,可能与OSAHS的昼夜血压变化有关 ,但在OSAHS并发高?更多还原

【Abstract】 Objective: To explore the relationship of the ACE gene insertion / deletion (I/D) polymorphism and the rennin-angiotensin system in the pathogenesis of hypertension in OSAHS. Method:Gene DNA was extracted from blood samples and amplified by polymerase chain reaction (PCR). The distribution of the ACE gene I / D allele and genotypes were analyzed in 30 OSAHS with hypertension patients, 30 normotensive OSAHS patients and 30 healthy control group without cardiovascular diseases. The serum level of angiotensin Ⅱ were also measured in all subjects by angiotensin Ⅱ enzyme immunoassay kit.Result:The frequency of II genotype and I allele were significantly higher in the OSAHS accompanied hypertension patients than those in the healthy controls (χ 2 = 9.88 , χ 2= 16.13 , P< 0.01 , respectively) and in the normotensive OSAHS patients (χ 2= 5.67 , P< 0.05 , χ 2= 8.61 , P< 0.01 ) .The frequency of II genotype and I allele had no significantly different between the normotensive OSAHS patients and the healthy controls (P> 0.05 ). The serum level of angiotensin Ⅱ was significantly higher in both OSAHS patients with and without hypertension than that in the healthy control (t= 3.66 , t= 3.23 , respectively P< 0.01 ). Whereas the serum level of angiotensin Ⅱ have no significantly different in the OSAHS patients with and without hypertension (P> 0.05 ). Conclusion: These results indicate that the higher frequency of ACE gene I allele and genotype are closely associated with OSAHS patients accompanied hypertension.It is one of the important risk factors for the genesis of hypertension in OSAHS.The activity of rennin-angiotension system is higher in the OSAHS may have contribute to OSAHS-induced hypertension.更多还原