【摘要】 目的 :探讨糖化血红蛋白 (Hb Alc)的检测在老年 型糖尿病 ( - DM)中应用的意义 ,及两种 Hb Alc测定方法的效果比较。方法 :高效液相色谱法 (HPL C)用弱酸阳离子交换树脂 ,在不同浓度洗脱液的离子强度及 p H条件下 ,将 Hb各组分分离。并与免疫比浊法比较。结果 :90例 Hb Alc的平均值为 :HPL C法 (7.85± 0 .4 4 ) % ,免疫比浊法 (7.93± 2 .4 5 ) % ,回归方程为 Y =0 .4 8+0 .95χ,γ =0 .97,Tγ =38.1,P <0 .0 1。结论 :两种方法呈良好相关性。均有较好的准确度及灵敏度 ,而免疫比浊法具有操作简便 ,实验速度快等特点更多还原

【Abstract】 Objective: To evaluate effects of HbA1c detection in the elderly with type Ⅱ diabetes mellitus and compare the two methods of HbA1c detection.Method: The HPLC method is that the Hb of each group was separated with weak acid positive ion exchange resin under the conditions of different density and pH of wash liquid Result: Average values of HbA1c in 90 cases were HPLC (7.85±0.4)%,and immune turbidimetry (7.93±2.45)%, respectively,compared with immune turbidimetry. The linear regressin equation was Y=0.48+0.95x, r=0.97,Tr=38.1,P<0.01.Conclusion: Both methods are accurate and sensitive. The immune turbidimetry is easy and quick.更多还原