【摘要】 目的 :比较ras基因在宣威、昆明两地肺癌中遗传变异的差异性 .方法 :采用免疫组化染色和PCR -SSCP -DNA直接测序技术 ,对 4 5例宣威地区肺癌和 4 5例昆明地区肺癌石蜡包埋组织标本中ras蛋白的表达及k -ras基因 12密码子突变进行检测 .结果 :5 5例 (5 5 / 90 )ras蛋白表达阳性 ,其中宣威肺癌组 31例 ,昆明肺癌组 2 4例 ,2 8例 (2 8/ 90 )检测到k -ras基因 12密码子突变 .对ras基因在两组肺癌中的表达及突变进行比较分析表明 :ras蛋白表达及k -ras基因 12密码子突变在宣威肺癌组中高于昆明肺癌组 ,但差异无统计学意义 (P >0 0 5 ) ,ras蛋白在Ⅰ期肺癌病例以及腺癌病例中的表达 ,宣威肺癌组均高于昆明肺癌组 ,差异有统计学意义 (P =0 0 4 5 ,χ2 =4 5 72 ,P =0 0 32 ) .结论 :ras基因的激活是肺癌发生过程中常见的分子生物学事件 ;宣威地区肺癌与昆明地区肺癌相比 ,ras蛋白在Ⅰ期病例以及腺癌病例中的表达存在着差异 .k -ras基因 12密码子在宣威地区肺癌中的突变与昆明地区肺癌相比无明显特异性更多还原

【Abstract】 Objective: To compare the differences of ras gene alternations in non-small-cell lung cancer (NSCLC) between Xuanwei and Kunming . Methods: Samples of paraffin-embedded lung cancer tissues of 45 patients from Xuanwei and 45 patients from Kunming with NSCLC underwent radical resection were analyzed for expression of ras protein and mutations of k-ras codon 12 by immunohistochemica staining, polymerase chain reaction-single strained conformation polymorphism and DNA detect sequencing. Results: Among the 90 cases, 55 cases had ras protein expression and 28 cases had k-ras codon 12 mutations. Comparing the alternations of ras gene in 2 groups of NSCLC, the frequancy of ras protein expression and of k-ras mutation in Xuanwei group were higher than that in Kunming group, but no statistically significant difference was found. Except that there were some statistically significant differences between 2 groups in terms of expression of ras protein in the cases with stage I and the cases with adenocarcinoma, but no statistically significant difference in k-ras codon 12 mutation was found between 2 groups. Conclusion: These data suggest that the activation of ras gene is a commonly genetic event in the pathogenesis of NSCLC, the mutations of k-ras gene codon 12 are ubiquitous in 2 groups of NSCLC, there was statistically signifimant difference in ras protein expression in patients with stage I and with ademocarcinoma between 2 groups.更多还原