【摘要】 观察痰瘀同治方对实验性动脉粥样硬化家兔主动脉、心肌及内皮细胞形态学的影响。采用高脂饲料制造家兔动脉粥样硬化模型 ,再给痰瘀同治方大、中、小剂量治疗 ,观察各组主动脉及心肌变化。之后体外内皮细胞培养 ,以高脂血清损伤内皮细胞 ,再给痰瘀同治方及不同剂量冠心Ⅱ号方 ,观察内皮细胞形态学变化。结果发现 ,扫描电镜下 ,痰瘀同治方大剂量组主动脉内膜较光滑 ,主动脉硬化斑块的形成明显减少 ,内皮细胞排列较密集规整 ,细胞间隙未见开大 ,细胞形态恢复 ,多为长梭形 ,其内膜表面的变化基本趋向正常。透射电镜下 ,痰瘀同治方组大剂量组心肌肌原纤维排列较规整 ,肌节排列亦较为整齐 ,明暗带分明 ,未见肌丝溶解 ,肌浆网扩张明显减少。倒置相差显微镜显示 ,痰瘀同治方小剂量组内皮细胞形态规整 ,细胞碎片减少 ,呈多角形和短梭形。荧光显微镜下内皮细胞呈淡蓝色椭圆形 ,染色均匀 ,亮度一致 ,可见少数凋亡及坏死的红染细胞。电镜下痰瘀同治方小剂量组内皮细胞形态与正常组相似 ,表面粗糙 ,微绒毛变短。结果提示 ,痰瘀同治方有明显减轻主动脉粥样硬化及改善心肌缺血缺氧状态 ,对血管内皮及细胞损伤有明显的保护作用更多还原

【Abstract】 Aim To observe the effect of Recipe of Removing Both Phlegm and Blood stasis on aorta and cardiac muscle of rabbits as well as ultrastructure of endothelial cells. Methods The rabbits were fed with high fat diet in order to reproduce hyperlipemia models; The endothelial cells were cultured in high fat serum to observe the effect of hyperlipemia on it. They were treated by two kinds of recipes. In this experiment the ultrastructure of endothelial cells (EC), aorta and cardiac muscle was observed through electron microscope. Results Recipe of Removing Both Phlegm and Blood stasis group: Smooth tunica intima of aorta, little formation sclerosis plaque of aorta, dense and regular arrangement of endothelial cells, recovered cell appearance, regular arrangement of cardiac muscle, slight adipose tissue infiltration in cardiac muscle, no myofilament solution and decrease of expansion of sarcoplasmic reticulum. The results showed that recipe prevented EC, aorta and cardiac muscle from injury. Conclusion Recipe of Removing Both Phlegm and Blood stasis can be used against myocardial ischemia, prevent vasospasm, platelet aggregation and arteriosclerosis.更多还原