【摘要】 为研究细胞间粘附分子 1、血管细胞粘附分子 1和肿瘤坏死因子α在人正常冠状动脉与冠状动脉粥样硬化组织中的表达及病理学意义 ,采用免疫组织化学SP法 ,分别检测细胞间粘附分子 1、血管细胞粘附分子 1和肿瘤坏死因子α在人正常冠状动脉与冠状动脉粥样硬化组织中的表达情况。结果发现 ,细胞间粘附分子 1、血管细胞粘附分子 1和肿瘤坏死因子α在动脉粥样硬化组织中的内皮细胞、平滑肌细胞、巨噬细胞均有表达。三者在脂纹期的表达分别为 5 0 .0± 10 .9、5 1.8± 6 .0和 13.9± 2 .8,在纤维斑块期分别为 2 3.1± 7.3、37.2± 9.7和 2 3.0± 6 .0 ,在粥样斑块期分别为 17.5± 4 .9、18.6± 5 .5和 38.0± 10 .0 ,明显高于对照组 (2 .2± 1.4、2 .2± 1.2和 7.8± 2 .2 ,分别为P<0 .0 1)。细胞间粘附分子 1和血管细胞粘附分子 1与肿瘤坏死因子α呈正相关 (前者r=0 .344、P <0 .0 1,后者r=0 .5 2、P <0 .0 1)。实验结果提示 ,细胞间粘附分子 1和血管细胞粘附分子 1在内皮细胞、平滑肌细胞和巨噬细胞高表达可能参与动脉粥样硬化发生发展过程中的某些环节。肿瘤坏死因子α的表达与细胞间粘附分子 1和血管细胞粘附分子 1的表达及动脉粥样硬化病变程度具有相关性。更多还原

【Abstract】 Aim To estimate the expression and significance of intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and tumor necrosis factor-alpha (TNF-α) in human normal coronary artery and coronary atherosclerosis. Methods SP immunohistochemical technique was utilized to measure the expression of ICAM-1, VCAM-1, TNF-α in normal caromrlyartery and caronary atherosclerosis respectively. Analyses were performed on SPSS 10.0. Results The expressions of ICAM-1,VCAM-1and TNF-α were on endothelial cell, smooth muscle cell and macrophage of atherosclerosis. They were expressed individually as 50.0±10.9, 51.8±6.0, 13.9±2.8 in fatty streak period, 23.1±7.3, 37.2± 9.7 , 23.0±6.0 in fibrous plaque period, 17.5±4.9, 18.6±5.5, 38.0±10.0 in atheromatous plaque period. They were all significantly higher than those in healthy controls (all P<0.01). There was positive correlation in ICAM-1, VCAM-1 and TNF-α (r1=0.344, P1<0.01; r2=0.52, P2<0.01). Conclusion The expression of ICAM-1,VCAM-1and TNF-αin endothelial cell,smooth muscle cell and macrophage of experimental group implied they partaked the certain link of progression in atherosclerosis. Expression of TNF-αwas related to ICAM-1,VCAM-1 and degree of atherosclerosis.更多还原