【摘要】 为观察吸烟对大鼠脑血管内皮细胞超微结构、细胞间粘附分子 1表达的影响 ,建立大鼠吸烟动物模型 ,将 75只大鼠随机分为长期大量组、长期小量组、短期大量组、戒烟组和对照组 ,免疫组织化学和原位杂交结合显微图像分析系统检测大鼠脑血管内皮细胞细胞间粘附分子 1的表达 ;电镜观察内皮细胞超微结构的改变。结果发现 ,吸烟大鼠脑血管内皮细胞细胞间粘附分子 1表达增加 (P <0 .0 5 ) ,与吸烟量和时程存在剂量—效应关系 ;内皮细胞超微结构改变显著。戒烟鼠细胞间粘附分子 1表达下调 (P >0 .0 5 ) ,内皮细胞超微结构病理改变明显恢复。结果提示 ,吸烟致脑血管内皮细胞细胞间粘附分子 1表达上调可能是吸烟导致动脉粥样硬化的分子机制之一。更多还原

【Abstract】 Aim To investigate effect of cigarette smoking onmorphology and expression of intercellular adhesion molecule-1 (ICAM-1), ICAM-1 mRNA in vascular endothelial cells of rats’ brains and to further explore influence of cigarette smoking on atherogenesis. Methods 75 male Wistar rats were randomly subgrouped into long-term massive, short-term massive, long-term trifle, anti-smoking and normal control group. Immunohistochemistry technique, in situ hybridization technique and pathologic image analyzing system were used to determine the expression of ICAM-1, ICAM-1 mRNA in rats’ cerebral vascular endothelial cells. Results In contrast to normal control group, expression of ICAM-1, ICAM-1 mRNA in rats’ cerebral vascular endothelial cells increased in smoking (103.7±3.9 vs 99.7±7.1 vs 92.6±5.2 vs 94.8±5.1 vs 84.2±4.7 for the mean gray value of ICAM-1mRNA staining in normal control, anti-smoking, short-term massive, long-term trifle, long-term massive group, repectively; P<0.05), and ICAM-1, ICAM-1 mRNA were relevant to the duration and dose of smoking (P<0.05). Expression of ICAM-1 and ICAM-1 mRNA decreased after anti-smoking, and there was no significant difference compared with normal control group (P>0.05). The structure of endothelial cells changed greatly in smoking, and partly recovered after anti-smoking. Conclusion The upregulation of expression of ICAM-1 and ICAM-1 mRNA might be one of the important molecular mechanisms during the process of atherosclerosis in cigarette smoking rats.更多还原