【摘要】 Wolbachia是广泛分布于节肢动物生殖组织内的一类细胞内共生菌 ,它属于原细菌的α亚类 ,能够通过调控寄主的生殖活动而促进其在寄主种群中的扩散。通过对wsp基因的克隆及PCR_RFLP分析确定了Wolbachia在我国广赤眼蜂种群内的存在 ,并发现有 2种Wolbachia菌系的感染 ,命名为wEvaA和wEvaB。经过克隆分离得到了这 2种Wolbachia的wsp基因序列 ,在GenBank的登录号为AY3 90 2 79和AY3 90 2 80 ,并由基于wsp基因的聚类树中发现 ,这两种Wolbachia菌系均属于A组更多还原

【Abstract】 Wolbachia, belonging to α-Proteobacteria, is a commonly widespread group of intracellular symbiotic bacteria found in reproductive tissues of arthropods. The bacteria may spread in host populations through regulation of host reproduction. In this study, the presence of Wolbachia was detected in a natural population of Trichogramma evanescens in China based on the wsp gene cloning and PCR-RFLP analysis. Two wsp genes of Wolbachia infecting T. evanescens were amplified and sequenced, with their GenBank accession numbers as AY390279 and AY390280. Also two new Wolbachia strains were identified and named as wEvaA and wEvaB. Through constructing and analysing the phylogenic tree of Wolbachia strains in Trichogramma spp. based on wsp gene, we found these two strains belonged to A group.更多还原