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九种蟋蟀mtDNA-16S rRNA基因序列及其系统进化(直翅目:蟋蟀科)

Phylogenetic Relationships of Nine Crickets Based on Partial Sequences of 16S rRNA Gene (Orthoptera:Gryllidae)

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【作者】 蒋国芳; 陈爱辉; 冯金叶;

【Author】 JIANG Guo-fang,CHEN Ai-hui,FENG Jin-ye(Jiangsu Key Laboratory for Bioresource Technology,College of Life Sciences,Nanjing Normal University,Nanjing,Jiangsu 210097,China)

【机构】 南京师范大学生命科学学院江苏省资源生物技术重点实验室; 南京师范大学生命科学学院江苏省资源生物技术重点实验室 江苏南京210097; 江苏南京210097; 江苏南京210097;

【摘要】 蟋蟀科5属9个种的线粒体16SrRNA基因部分序列被测定或从GenBank获得,比较其同源性,计算核苷酸使用频率,并构建NJ和MP分子系统树。在获得的449bp的序列中A、T、C和G碱基含量分别为31.8%、36.9%、9.9%和21.4%,A+T平均含量为68.7%。研究结果表明:所研究的5属9种蟋蟀聚成3个聚类簇,斗蟋属先与灶蟋属汇合,再与棺头蟋属构成聚类簇I;油葫芦属黑脸油葫芦和北京油葫芦与蟋蟀属的家蟋相聚构成聚类簇II;蟋蟀属的田蟋单独构成聚类簇III。

【Abstract】 The partial sequences of mitochondrial 16S rRNA gene (449bp) were sequenced for 9 species of crickets belonging to 5 genera of Gryllidae. The homologous sequences were compared, the used frequency of nucleotide was calculated and the molecular trees reconstructed by Neighbor-Joining method and maximum parsimony method using Dianemobius taprobanensis as outgroup. In the obtained sequences of crickets, the content of A, T, C and G was 31.8%, 36.9%, 9.9% and 21.4% respectively, and A% + T% was 68.7%. The reseach results suggested that 9 individuals from the 5 genera clustered in 3 clusters, among which genus Velarifictorus and Gryllodes firstly clustered and then together with genus Loxoblemmus to form cluster I; Cluster II was made of three species, Teleogryllus emma, T. mitratus and Gryllus domesticus; G. campestris single as cluster III.

【关键词】 直翅目; 蟋蟀科; 16SrRNA基因; 系统进化;
【Key words】 Orthoptera; Gryllidae; 16S rRNA gene; phylogeny;
【基金】 国家自然科学基金资助项目(30160015)
  • 【文献出处】 昆虫分类学报 ,Entomotaxonomia , 编辑部邮箱 ,2004年04期
  • 【分类号】Q963
  • 【被引频次】22
  • 【下载频次】317
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