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丹参药材中丹酚酸B的提取及其HPLC法测定

Extraction of Salvianolic Acid B from Danshen (Salvia Miltiorrhiza Bge. ) and Determination by HPLC Method

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【作者】 王凤美李磊柳先平王小如黎先春

【Author】 WANG Feng-mei,LI Lei,LIU Xian-ping,WANG Xiao-ru,LI Xian-chun(The Key Laboratory of Analytical Science of Ministry of Education , Department ofChemistry ,Xiamen University , Xiamen , 361005; First Institute of Oceanography,State Oceanic Administration , Qingdao , 266061)

【机构】 厦门大学化学化工学院 现代分析科学教育部重点实验室国家海洋局第一海洋研究所国家海洋局第一海洋研究所 福建 厦门 361005山东 青岛 266061山东 青岛 266061

【摘要】 目的 建立丹参中丹酚酸B的优化提取条件,并确立其HPLC测定方法。方法 丹参粉末在适当的溶剂中冰浴超声,提取物进行HPLC分析。色谱柱为Alltima C18(5μm,250 mm×4.6 mm);流动相为乙腈-0.5%冰醋酸水溶液,采用梯度洗脱程序,流速为1.0 mL/min;紫外检测波长为290 nm。结果 在冰浴超声条件下,70%甲醇对丹酚酸B提取率最高。水的提取率为70%甲醇的95.06%。丹酚酸B在0.07~0.70μg范围内呈良好的线性关系(r=0.9997)。精密度实验中RSD为1.62%,稳定性和重现性实验中RSD均小于3%。结论 本实验提取丹酚酸B处理方法简单,测定结果稳定可靠,可作为丹酚酸B含量测定方法。

【Abstract】 Objective To optimize the conditions for extracting Salvianolic acid B from Danshen (Salvia miltiorrhiza Bge. ). Methods Danshen crude drugs were dried, ground and sieved through 60-mesh screen sieve, followed by 20min ultrasonication in ice bath with different solvents. The Danshen suspensions were filtered and Salvianolic acid B in the filtrate was determined by HPLC. The column used was Alltima C18 (5μm, 250 mm×4. 6 mm); The mobile phase was with acetonitrile-0. 5% acetic acid solution in program of elution; the flow rate was 1. 0 mL/min and the detect wavelength was 290 nm. Results With ice bath, 70% methol achieved the highest rate of extraction, and with-water extraction was 95. 06% of that with 70% methol. Repeatability and precision were good. Salvianolic acid B had a good linearity within the range of 0. 07-0. 70μg (r=0. 9997),and the RSD was less than 3%. Conclusion The treatment of samples was simple and the results were accurate. This method may be used for extraction and determination of Salvianolic acid B.

【关键词】 丹酚酸B提取高效液相色谱法
【Key words】 Salvianolic acid BextractionHPLC
【基金】 国家自然科学基金重点项目(20235020)青岛市重点实验室建设项目(04-2-JS-135)资助
  • 【文献出处】 现代中药研究与实践 ,Research and Practice of Chinese Medicines , 编辑部邮箱 ,2004年S1期
  • 【分类号】R284.1
  • 【被引频次】65
  • 【下载频次】1401
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