Objective To establish a HPLC method to determine the content of plantagoside inPlantago major. Which is used most areas of China as the sample Semon Plantgo. Method : A hypersil C18 column (4. 6×250 mm i. d. , 5 μm) was used with 1. 0 mL. min 1 methanol-water-acetic acid(40 : 60 : 0. 5) as mobile phase. Detection was performed with UV detector at 288nm. Result Good linearity of the calibration curve between the peak area and the concentration of plantagoside was obtained in the range from 0. 49-2. 47 μg(r ...