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薄层色谱指纹图谱在甘草GAP生产与质控中的应用
The Application in GAP Production and Quality Control of glycyrrhiza uralensis Fisch.by TLC Fingerprint Spectrum
【摘要】 目的 研究甘草药材的薄层色谱指纹图谱 ,为科学评价及有效控制其质量提供可靠方法。方法 样品经预处理后点于硅胶 GF2 54预制板上 ,以乙酸乙酯 -乙酸 -甲酸 -水 ( 15∶ 1∶ 1∶ 2 )为展开剂 ,于 2 5 4 nm处进行吸收扫描。结果 甘草药材样品得到的薄层色谱指纹图谱有 6个共有峰 ,依此建立的柱状条形码图能快速地对药材品质作出评判。结论 采用 TL C指纹图谱可有效控制 GAP基地甘草的质量。
【Abstract】 Objective To set up a sensitive and specific TLC method for controlling the quality of glycyrrhiza uralensis Fisch..Method The sample solution was applied to the commercial pre-coated 20×10 cm silica gel F 254 plate (Merck) modified by 1% sodium hydroxide, ethyl acetate-formic acid-ethanoic acid-water(15∶1∶1∶2) as the solvent system, and quantitative analysis was carried out using UV absorptive scanning at 254nm. Result The TLC-FP of glycyrrhiza uralensis Fisch.showed 6 characteristic common peaks. The pole graph based on the height of common peaks can be conveniently used to evaluate the quality of different samples. Conclusion Quality of GAP glycyrrhiza uralensis Fisch. can be controlled effectively by TLC fingerprint.
【Key words】 Glycyrrhiza uralensis Fisch; Thin layer chromatography; Fingerprint; GAP;
- 【文献出处】 现代中药研究与实践 ,Research and Practice of Chinese Medicines , 编辑部邮箱 ,2004年04期
- 【分类号】TQ461
- 【被引频次】34
- 【下载频次】591