【摘要】 目的　研究晚期糖基化修饰白蛋白 (AGEmodifiedbovineserumalbumin ,AGE -BSA)撤退对体外培养牛视网膜内皮细胞 (bovineretinalendothelialcell,BREC)内吞活性及细胞内信号传递的影响。方法　BREC事先在AGE -BSA下持续作用 2 4h ,研究金雀异黄素 (genistein ,Gen)对AGE -BSA撤退后 12h和 2 4h的BREC内吞活性以及eNOS活性和NO浓度的变化的影响。结果　AGE -BSA撤退 12h和 2 4h后BREC内吞、eNOS活性和NO的浓度下调 ,Gen在对以上变化没有明显的影响。结论　AGE -BSA撤退过程中BREC内吞的变化不依赖于酪氨酸蛋白激酶活性物 ,而直接或间接与eNOS -NO系统相联系更多还原

【Abstract】 Objective To study the endocytosis and signal transduction of bovine retinal endothelial cell (BREC)after being withdrawn AGE modified bovine serum albumin(AGE-BSA). MethodsBREC was operated by AGE-BSA for 24h in advance. The endocytosis, eNOS activity and NO concentration of BREC applying with genistein was studied after 12h and 24h withdrawn AGE-BSA.Results The endocytosis, eNOS activity and NO concentration of BREC was down-regulated after withdrawn AGE-BSA. Genistein has no remarkable effective with the changes hereinbefore. Conclusions The endocytosis of BREC was independent of protein tyrosine kinase, but has relationship with eNOS-NO system directly of indirectly.更多还原