【摘要】 目的　建立清脑宣窍方有效部位中藏红花酸的含量测定方法。方法　采用反相高效液相色谱 (RP HPLC)法测定藏红花酸含量 ,运用YWG C18色谱柱 (2 5 0mm× 4 6mm ,10 μm) ,流动相为甲醇-水 -冰乙酸 ,流速 :1mL min ,检测波长 :4 2 3nm。结果　藏红花酸在 0 0 2 2～ 0 110 μg范围内与其色谱峰面积呈良好的线性关系 ,r =0 9991,平均回收率为 99 4 % ,RSD =1 90 % (n =5 )。结论　本法操作简便、准确 ,可作为清脑宣窍方有效部位的质量控制方法之一。更多还原

【Abstract】 Objective To establish a method for the quantitative determination of crocetin in the effective fraction of Qingnaoxuanqiao Formula (QF) by RP-HPLC. Method The RP-HPLC technique was used in the experiment. YWG-C 18 column (250 mm×4.6 mm, 10 μm) was used, the mobile phase was methanol-water-glacial acetic acid (83:17:34) at a flow speed of 1 mL/min, and the detection wavelength was 423 nm.Result Crocetin showed, between 0.022～0.110μg, a fine linear relationship with the peak areas (r=0.9991). The average recovery of this procedure was 99.4%, with RSD equal to 1.90% (n=5).Conclusion This procedure is easy and accurate, and can be used as one of the methods for controlling the quality of the effective fraction of QF.更多还原