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蛋鸡血清载脂蛋白AⅡ的分离纯化及初步鉴定
Isolation, Purification and Primary Characterization of Serum Apolipoprotein AⅡin Laying Hen
【摘要】 介绍了一种在普通实验室条件下,分离纯化蛋鸡血清载脂蛋白AⅡ的一种简便易行的方法。用磷钨酸镁沉淀分离出蛋鸡血清HDL。乙醇/丙酮(V/V=1∶1)脱脂后经DEAESepharoseTMCL-6B和SephadexG-150柱层析。获得的apoAⅡ经15%的尿素-SDS-PAGE电泳显示为单一条带。分子量测算大约为8673da。本方法的优点是通过使用易行的磷钨酸镁沉淀法代替了超速离心的复杂过程。不需特殊仪器,缩短了制备时间且分离效果理想。
【Abstract】 Apolipoprotein AⅡof laying hen was isolated,purified and primarily characterizated in the common laboratory. HDL from laying hen was isolated and purified from pooled serum by precipitating with phosphotungstic acid-Mg, apolipoprotein AII was obtained from HDL which was delipitated with Ethanol/Acetone（V∶V =1∶1）and permeated chromatography on DEAE sepharoseTM Cl-6B column and sephadex G-150 column ,primarily characterizated by 15% Urea-SDS-PAGE.The purified apolipoprotein AII showed only one band on Urea- SDS-PAGE and the estimated molecular weight is 8673da.The advantage of this method is that by precipating with phosphotungstic acid-Mg instead of ultracentrifugation, which is very complicated,it does not need special instruments; at same time, time is saved but the effect is satisfactory.
【Key words】 laying hen； serum； high density lipoprotein(HDL)； apolipoprotein AII(apoAII)； precipitate with phosphotungstic acid-Mg； electrophoresis； permeation chromatography；
- 【文献出处】 江西农业大学学报 ,Acta Agriculturae Universitis Jiangxiensis , 编辑部邮箱 ,2004年06期
- 【分类号】S852.5
- 【被引频次】1
- 【下载频次】121