【摘要】 采用在培养基中添加重稀土元素铒(Er3+)的方法制备了稀土铬酵母,研究了在培养基麦芽汁中Er3+的质量分数分别为0 00050%、0 00075%、0 0010%和0 00125%时对铬酵母中铬含量的影响。研究结果表明,Er3+能够有效提高铬酵母中总铬和有机铬的含量,当在培养基中添加0 0010%Er3+时,所得稀土铬酵母中的总铬和有机铬含量最高,其质量分数分别达到了0 054%和0 051%,与普通铬酵母相比,其有机铬含量提高了3倍以上。通过紫外光谱分析,发现铬酵母在260nm处有特征吸收峰,而加Er3+的铬酵母样品在此处的吸收峰显著增强,说明稀土铬酵母中葡萄糖耐量因子的含量显著增加。更多还原

【Abstract】 Rare earth chromium yeasts were cultured with Cr³⁺ and different mass fractions（0.00050%,0.00075%,0.0010% and 0.00125%） of Er³⁺ added in the medium.It was found that Er³⁺ could effectively increase the mass fractions of total chromium and organic chromium in the chromium yeast.When 0.0010% Er³⁺ was added in the culture medium,the mass fractions of total chromium（0.054%） and organic chromium（0.051%） in the cultured rare earth chromium yeast were both the highest.Content of organic chromium was more than three times that in the chromium yeast without rare earth in the culture medium.By ultraviolet analysis,it was found that the absorption peak of organic chromium at 260 nm was markedly enhanced in the sample added Er³⁺,which indicated that the mass fraction of glucose tolerance factor in the rare earth chromium yeast was increased.更多还原