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籼稻矮泰引-3矮生性的遗传分析与半矮秆基因的鉴定

Genetic Analysis of the Dwarfism and Identification of the Semidwarf Genes in an Indica Rice Aitaiyin-3 ( Oryza sativa L.)

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【作者】 赵祥强周劲松梁国华严长杰曹小迎顾铭洪

【Author】 ZHAO Xiang-qiang, ZHOU Jing-song, LIANG Guo-hua, YAN Chang-jie, CAO Xiao-ying, GU Ming-hong ( Agric Coll, Yangzhou Univ, Yangzhou, 225009, China)

【机构】 扬州大学农学院扬州大学农学院 江苏扬州225009江苏扬州225009

【摘要】 为研究籼稻特矮生材料矮泰引 3的矮生性遗传,将其与高秆品种南京6号杂交,杂种F1表现为高秆,F2群体中高秆个体、半矮秆个体和矮秆个体的分离比例符合9∶6∶1的分离比,表明矮泰引 3的矮生性状受2对独立遗传的隐性主效半矮秆基因控制。选取F2群体中的半矮秆个体与南京11号测交,根据测交结果将与sd 1不等位的半矮秆株系鉴别出来,并将新的矮秆基因暂命名为sd t2;将携带sd t2的半矮秆株系与南京6号回交,BC1F2的分离情况佐证了sd t2的遗传效应的确表现为半矮秆。对BC1F2中分离出的3个半矮秆株系的种子进行赤霉素(GA3)溶液的苗期处理,幼苗生长状况表明sd t2对外源GA3处理敏感。

【Abstract】 Aitaiyin-3, a natural rice dwarf mutant originated from Taiyin 1, was crossed with a tall cultivar Nanjing 6. The F1 individuals were as tall as the taller parent and the segregation of the F2 generation appeared to be 9 tall∶6 semidwarf∶1 dwarf, which meant the dwarfism of Aitaiyin-3 was controlled by two recessive semi-dwarf genes without linkage. Nanjing 11, a semi-dwarf cultivar carrying sd-1 , was used as the tester to be crossed with the semidwarf individuals selected from the F2 population. The allelism test indicated that the one semidwarf gene of Aitaiyin-3 was an allelic to sd-1 and the other was a new one nonallelic to sd-1 . Then the semidwarf F3 lines with the new semidwarf gene were identified and backcrossed with Nanjing 6. The approximately segregation ratio of BC1F2 populations was 3 tall∶1 semidwarf and the semidwarfing effect of this new gene was confirmed. This gene was named as sd-t2 temporarily, since a semidwarf gene sd-t had been identified from Aitaiyin-2 which also could be traced back to Taiyin-1. At the same time, three semidwarf plants in the BC1F2 population from Nanjing 6 background were selected randomly and their seeds were treated with gibberellin (GA3), contrasted with Nanjing 11 and Aitaiyin-3. The result showed sd-t2 was sensitive to exogenous GA3.

【关键词】 水稻半矮秆基因分离遗传赤霉素
【Key words】 ricesemidwarf geneisolationinheritancegibberellin
【基金】 国家自然科学基金资助项目(39900086);国家重点基础研究发展规划项目(973G1999011606);江苏省自然科学基金资助项目(BK2002046)
  • 【文献出处】 扬州大学学报 ,Journal of Yandzhou University , 编辑部邮箱 ,2004年01期
  • 【分类号】S511.21
  • 【被引频次】14
  • 【下载频次】92
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