【摘要】 目的 :观察比较猪下腔静脉 (IVC)血栓形成后第 1、4、7天内皮细胞表达IL 1、TNF α、P selectin含量的变化。方法 :取仔猪 30头 ,15头进腹结扎肾下段IVC制成血栓模型为实验组 ,5头开腹不结扎IVC为对照组。待形成血栓模型后第 1天 (A组 )、第 4天 (B组 )和第 7天 (C组 )取出结扎段静脉标本 ,以酶解法收集内皮细胞 ,采用半定量RT PCR法检测细胞内IL 1,TNF α及P selectin表达变化情况。结果 :酶解法收集的内皮细胞纯度达 99.4 2 %± 0 .0 7%。TNF α在A组即有表达 ,B组达高峰 (P <0 .0 5 ) ,C组则降低 ;P selectin的表达随时间的延长而逐渐升高 (P <0 .0 5 ) ;IL 1只在A组表达 ;上述三种炎症因子在对照组均不表达。结论 :(1)内皮细胞不仅是炎症介质的靶细胞 ,也能自主表达多种活性因子促进静脉血栓形成。 (2 )IL 1mRNA仅在血栓形成早期短暂表达 ;TNF αmRNA表达在血栓形成早期逐渐增强 ;P selectinmRNA表达在血栓形成急性期呈逐渐增强的趋势。更多还原

【Abstract】 Objective:Experiments were designed to compare the expressions of IL-1 TNF-α P-selectin mRNA by porcine endothelial cells after vein thrombosis.Methods:IVC under the renal vein of 20 porcines were ligated to induce thrombosis modes.These thrombosed veins were divided into three groups:group A-one day after thrombosis,group B-four days after thrombosis and group C-seven days after thrombosis.The other pigs were given the shame operation as a contro group (group D).The mRNA levels of IL-1 TNF-α and P-selectin expressed by porcine endothelial cells in three groups were analysed by semiquantitative RT-PCR.Endothelial cells were harvested with collagenase Ⅱ.Results:The purity of endothelial cells harvested was 99.42%±0.07%.The expression of IL-1 was detained only in group A while TNF-α reached its peak in group B(P<0.05) and P-selectin increased gradually with the days passing by (P<0.05).Conclusion:(1)Not only are endothelial cells the targets of inflammatory factors,but also they can express some activator factors in vein thrombosis.(2)Expression of TNF-α mRNA is increased gradually in the early period of vein thrombosis while P-selectin in the acute period.Expression of IL-1 mRNA is transcient.更多还原