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微胶囊法和交联法固定化磷脂酶A1的比较
Comparing the methods of microcapsule and covalention on the immobilizied phospholipase A1
【摘要】 采用不同固定化磷脂酶A1的方法,即交联法和微胶囊法,以壳聚糖和海藻酸钠为载体,戊二醛为交联剂,固定化磷脂酶A1.通过考察固定化载体和固定化酶的性质以及固定化酶的活力回收等,比较两种固定化酶方法的优劣.结果表明,微胶囊包埋法固定化磷脂酶A1工艺较佳.
【Abstract】 Using alginate and chitosan as carrier of immobilizied enzyme, glutaradehyde as (crosslin-)(king) agent, phospholipase A1 (PLA1) was immobilizied by the methods of covalention and microcapsule. The characteristics of carrier and immobilizied PLA1 and recovery of enzyme activity are studied, in order to compare two ways of immobilizied PLA1. The results showed that the method of microcapsule for immobilizied PLA1 was the optimum way.
【基金】 国务院侨办重点资助项目(93A119)
- 【文献出处】 暨南大学学报(自然科学与医学版) ,Journal of Jinan University(Natural Science & Medicine Edition) , 编辑部邮箱 ,2004年05期
- 【分类号】O629
- 【被引频次】16
- 【下载频次】481