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斑点免疫金银试验(Dot-IGSS)检测牛结核血清抗体的研究
Study on Detection of Tuberculosis Antibodies in Serum of Cattle by Dot-IGSS
【摘要】 建立了斑点免疫金银试验(Dot—IGSS)检测牛结核血清抗体的方法。应用此方法检测141头份结核变态反应阳性牛血清,其阳性检出率为64.5%(91/141),同时检测124头份变态反应阴性牛血清,其阳性检出率为14.5%(18/124)。经阻断试验、交叉试验及重复试验,证明了牛结核Dot—IGSS具有较好的特异性。
【Abstract】 We established Dot-IGSS (Dot-immunogold silver staining) to detect antibodies in sera of cattle contracting tuberculosis. By detecting 141 cattle whose tuberculosis allergic reaction detected by Bovine tuberculosis Dot-IGSS was positive, 64.5% (91/141) positive rate was obtained. By detecting 124 cattle whose tuberculosis allergic reaction was negative, 14.5% (187124)positive rate was obtained. The specificity of Dot-IGSS was confirmed by blocking and cross-reacting test.
【基金】 国家自然科学基金资助项目(30270986)
- 【文献出处】 吉林农业大学学报 ,Journal of Jilin Agricultural University , 编辑部邮箱 ,2004年02期
- 【分类号】S852.5
- 【被引频次】1
- 【下载频次】103