【摘要】 建立了斑点免疫金银试验(Dot—IGSS)检测牛结核血清抗体的方法。应用此方法检测141头份结核变态反应阳性牛血清,其阳性检出率为64.5％(91/141),同时检测124头份变态反应阴性牛血清,其阳性检出率为14.5％(18/124)。经阻断试验、交叉试验及重复试验,证明了牛结核Dot—IGSS具有较好的特异性。更多还原

【Abstract】 We established Dot-IGSS (Dot-immunogold silver staining) to detect antibodies in sera of cattle contracting tuberculosis. By detecting 141 cattle whose tuberculosis allergic reaction detected by Bovine tuberculosis Dot-IGSS was positive, 64.5% (91/141) positive rate was obtained. By detecting 124 cattle whose tuberculosis allergic reaction was negative, 14.5% (187124)positive rate was obtained. The specificity of Dot-IGSS was confirmed by blocking and cross-reacting test.更多还原