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血浆p16基因甲基化在原发性肝细胞癌诊断中的作用
Methylation of p16 gene in plasma in diagnosis of primary hepatocellular carcinoma
【摘要】 用甲基化特异性-多聚酶链反应(MSP)方法,检测原发性肝细胞癌(HCC)、肝硬化、慢性肝炎病人及健康人各30例的血浆DNA甲基化的p16基因.结果显示,HCC病人血浆p16基因甲基化率为67%,慢性肝炎、肝硬化及健康者未发生甲基化.
【Abstract】 Using the methylation specific PCR(MSP) to detect the methylation of p16 geng of each of 30 patients with primary hepatocellular carcinoma(HCC), 30 patients with cirrhosis, 30 patients with chronic hepatitis and 30 healthy individuals, respectively. The results show that the methylation of p16 gene was founded in 20 of the 30 patients (67%) with primary hepatocellular carcinoma. In the other groups was without the methylation of p16 gene.
【基金】 吉林省教育厅基金(批准号:200027).
- 【文献出处】 吉林大学学报(理学版) ,Acta Scientiarium Naturalium Universitatis Jilinensis , 编辑部邮箱 ,2004年02期
- 【分类号】R735.7
- 【被引频次】3
- 【下载频次】94