【摘要】 目的　探讨L 精氨酸对严重腹腔感染大鼠肠屏障功能的影响。方法　SD大鼠 90只 ,随机分成对照组、感染组和精氨酸组 ,每组 30只。对照组仅行单纯剖腹手术 ;感染组采用盲肠结扎加穿孔 (CLP)手术制作严重腹腔感染模型 ;精氨酸组行CLP后每天添加L 精氨酸饮食 [0 2 6 4g/ (kg·d) ]。各组术后 1、2、 4d分别取肠黏膜行病理学观察、增殖细胞核抗原 (PCNA)指数测定、血细菌培养和血浆内毒素水平测定。结果　精氨酸组较感染组小肠黏膜病理性损害明显减轻。感染组术后 2、 4d血浆内毒素水平明显高于精氨酸组 ,分别是 (1 4 2± 0 10 )EU/mlvs (1 14± 0 11)EU/ml,(1 94± 0 0 7)EU mlvs (1 5 8± 0 14 )EU/ml,P <0 0 1。精氨酸组术后 2d血细菌阳性率明显低于感染组 (10 %vs 30 % ,P <0 0 1)。感染组PCNA指数先升高 ,后下降 ,而精氨酸组 4d内下降不明显。结论　当严重腹腔感染导致肠屏障功能障碍时 ,L 精氨酸有促进肠黏膜损伤修复、维护肠屏障功能的作用更多还原

【Abstract】 Objective To explore the effect of L-arginine on gut barrier function in rats with severe abdominal infection.Methods A total of 90 SD rats were randomly divided into three groups(n=30 each): control group(laparotomy only), infection group(cecal ligadon plus puncture),and arginine group [0.264 g/(kg·d) of L-arginine added in the diet after cecal ligadon plus puncture]. The changes of intestinal mucosa pathology and PCNA index were observed.Blood culture and plasma endotoxin levels were taken at 1, 2 and 4 day after operation. Results The inflammatory reaction of intestine mucosa was more severe in the infection group than in the arginine group. The positive rate of bacteria culture and plasma endotoxin level were higher in the infection group than in the arginine group and the control group. The PCNA index rose initially, then declined gradually in the infection group, while in the arginine group PCNA indexes were continuously high.Conclusion When the gut barrier is disturbed during severe abdominal infection in rats,L-arginine can alleviate the repair of intestinal mucosa, and preserve the gut barrier function.更多还原