【摘要】 目的　检测犬心肺复苏后 6h ,肺组织TNF α、IL 6浓度和肺中TNF αmRNA、IL 6mRNA、IL 10 mRNA的表达变化 ,以探讨纳洛酮对肺组织中TNF α、IL 6、IL 10含量的影响。方法　 18只健康杂种犬 ,随机分成 3组 ,空白组 (n =6 ) :不诱发室颤 ,6h后取肺组织 ;对照组 (n =6 ) :心跳骤停后予常规心肺复苏术 ,实验组 (n =6 ) :心跳骤停后予常规心肺复苏术 +纳洛酮。于复苏后 6h取肺组织行TNF α、IL 6浓度和肺TNF α;mRNA、IL 6mRNA、IL 10mRNA的表达的测定以及行肺形态学检查。结果　实验组TNF α浓度明显低于对照组 (P <0 0 1) ,实验组、对照组、空白组IL 6浓度无差别 (P >0 0 5 )。实验组IL 10mRNA的表达明显高于对照组 (P <0 0 5 ) ,实验组和对照组的TNF αmRNA、IL 6mRNA的表达无差别 (P >0 0 5 )。实验组肺组织的病理损害低于对照组。结论　纳洛酮可减轻心跳骤停后肺组织的TNF α的生成 ,使IL 10mRNA的表达增加 ,从而减轻心肺复苏后肺的再灌流损伤。更多还原

【Abstract】 Objective To observe influence of concentration of lung TNF-α, IL-6 and the expression of lung TNF-α,IL-6 and IL-10 6 hour after cardiopulmonary resuscitation(CPR) in dogs.Methods Group 1(n=6)lung tissue was taken without cardiac arrest after 6 hour. Group 2 (n=6) was performed standard CPR after cardiac arrest in dogs. Group3 were group 2+naloxone. Lung tissue was taken out 6 hours after CPR. The concentrations of lung TNF-α,IL-6 and the expressions of lung TNF-α, IL-6 and IL-10 were measured and lung tissue was also used for histological examination.Results The concentration of lung TNF-α in group 2 was significantly higher than in group 3(P<0.01)and group 1(P<0.01).The mRNA expression of lung IL-10 in group 3 was significantly highter than in group 2(P<0.05) and in group I(P<0.01).The mRNA expressions of lung TNF-α,IL-6 in three groups were not statistically different.Pathological lung injury in group 2 was significantly lower than in group 3. Conclusion Natoxone can attenuate ischemia reperfusion lung injury after CPR through reducing TNF-α and increasing the mRNA expression of lung IL-10.更多还原