【摘要】 采用PCR克隆技术对黑熊四川亚种线粒体DNA cytb基因进行克隆、测序。黑熊四川亚种线粒体DNA cytb基因的全序列为1140 bp,CG含量为46.23％。与AB020910和U23558两个黑熊cytb序列的变异共有61处.其中T-C转换共有36处.A—G转换共有23处。仅有2处发生T-A颠抉,碱基突变率为5.35％.其中转换率96.72％,颠换率为3.28％。密码子第3个碱基发生变异的有48个。更多还原

【Abstract】 An Asian black bear (Ursus thibetanu mupinensis) mtDNA Cytb gene was amplified, cloned and sequenced. The full length of the black bear mitochondrial DNA Cytb gene (1140bp) is obtained, which C + G content accounts for 46.25 % . The comparison of the nucleotide sequence obtained in this experiment among the sequences downloaded from GenBank( AB020910 and U235S8) showed 61 mutations , including 36 transpositions of T - C, 23 A - G and 2 T - A. The rate of base mutation and transposition of T-C ,A-G&T-Ais5.35% and 96.72 % , 3.28 % respectively. 48 mutation sites were found at the 3rd of base of codons.更多还原