【摘要】 目的 :比较使用激光和机械两种方法制动睾丸精子后行卵母细胞内单精子注射的受精率、卵裂率和优质胚胎形成率的异同 ,探索激光辅助法精子制动在睾丸精子卵母细胞内单精子注射中应用的可能性。方法 :1 6对外周血染色体正常、无体外受精 -胚胎移植禁忌症的无精子症夫妇 ,女方用GnRH Gonal-F HCG长方案超数排卵 ,将每对夫妇获得的卵用透明质酸酶去除颗粒细胞后随机均分为 2组 ;女方采卵当日丈夫睾丸取精处理后分别用激光法和机械法制动精子并行卵母细胞内单精子注射 ,2 4小时观察受精卵数目 ,48小时观察卵裂受精卵的数目和优质胚胎的数目并计算出两组各自的受精率、卵裂率和优质胚胎形成率进行比较研究。结果 :1 6对夫妇 1 6个周期共获得MⅡ期卵母细胞 1 89枚。激光制动组 ( 95枚 )的受精率为 71 .6 % ( 6 8 95 ) ,卵裂率为 6 0 .0 % ( 5 7 95 ) ,优质胚胎形成率为 5 3 .7% ( 5 1 95 ) ;机械制动组 ( 94枚 )的受精率为70 .2 % ( 6 6 94) ,卵裂率为 6 1 .7% ( 5 8 94) ,优质胚胎形成率为 5 2 .1 % ( 49 94)。两组相比受精率、卵裂率和优质胚胎形成率均无统计学差异 (P >0 .0 5 )。结论 :激光辅助法精子制动可以应用于睾丸精子卵母细胞内单精子注射更多还原

【Abstract】 Objective:To Compare the difference in rates of fertilization,cleavage,and good quality embryos between laser assisted immobilization and mechanical immobilization,for the purpose of demonstrating the possibility of using laser assisted immobilization before intracytoplasmic sperm injection.Methods:This study includes in 16 couples with normal chromosome karyotypes of peripheric lymphocyte,and without contradict signs to ICSI-ET.GnRH/Gonal-F/HCG protocol are performed for oocytes collection in women,total 189 MⅡ oocytes in 16 cycles are divided into two groups equally at random;testicular biopsies for sperms are done in men and the sperms collected were immobilized by laser assisted or mechanical method before intracytoplasmic sperm injection.After 24h,48h the numbers of fertility,cleavage and good quality embryos recorded;and ratos are calculated and analyzed in each group respectively.Results:It has shown that the rates of fertility,cleavage,and good quality embryos are 71.6%(68/95),60.0%(57/95)and 53.7%(51/95)in laser assisted immobilization group;and 70.2%(66/94),61.7%(58/94)and 52.1%(49/94)in mechanical immobilization group correspondently and those rates have no significant difference between two groups statistically(P>0.05).Conclusions:Laser assisted sperm immobilization is simple to manipulate,and easy to control.It is useful and effective to use laser assisted testicular sperm immobilization before intracytoplasmic sperm injection and embryo transfer.更多还原