【摘要】 测定了八棱丝瓜蛋白1的N端顺序为Asp-Val-Ser-Phe-Ser-,用CD谱测定了八棱丝瓜蛋白 1的α螺旋,β-折叠和无规卷曲含量分别为37.1%,33.4%,29.5%。实验表明,八棱丝瓜蛋白 1具有RNA N-糖苷酶活性,体外抑制肿瘤细胞生长活性表明其对肿瘤细胞株B16,MGC,Bel的半数抑制浓度分别为1.78×10-7mol/L,2.11×10-7mol/L和4.21×10-7mol/L。并在N端顺序,二级结构和生物活性方面对八棱丝瓜蛋白 1和天花粉蛋白进行了比较。更多还原

【Abstract】 The N-terminal sequence of Luffaculin 1 was determined to be Asp-Val-Ser-Phe-Ser- . The CD spectrum of Luffaculin 1 indicated that Luffaculin 1 contains the 37.1%α-helix , 33.4% β-sheet,and 29.5% random coil. N-glycosidase activity of Luffaculin 1 against animal rRNA is observed. The anti-tumor activity of Luffaculin 1 on cell strains B16, MGC, Bel were determined,giving IC50 of 1.78×10-7mol/L,2.11×10-7mol/L,and 4.21×10-7mol/L,respectively. We also discussed the N-terminal sequences, secondary structures, and bioactivities of Luffaculin 1 against Trichosanthin.更多还原