【摘要】 目的　通过对聚氨酯材料的细胞毒性研究 ,评价其作为人工心脏辅助泵体材料的可行性。方法　采用材料直接接触细胞培养法和四唑盐(MTT)比色试验评价聚氨酯的细胞毒性。结果　聚氨酯材料直接接触L 92 9细胞后具有良好的细胞相容性。MTT比色试验证明聚氨酯材料具有较低的OD值。结论　聚氨酯材料具有良好的细胞相容性。更多还原

【Abstract】 Objective To study the cytotoxicity of the membrane material of heart assist pump. Methods The membrane materials tested incladed polyurethane (group A) and medical polychloroethylene (group B),and a blank control group with none of the material. L-929 cells (the mouse fibroblasts) were cultured on either material in groups A and B,and 6 cell cultures were without any material. After 24,48 and 72 hours,the growth of cell in groups A,B and the control was observed,and the number was counted. Educts of two materials used in groups A and B was respectively added to culture medium,then the OD was measured every 24h and proliferation rate was determined by MTT test. Results L-929 cells grew very well in groups A and B with less death. The cell counts showed no difference between 2 groups at each time. The OD of group A was always higher than that of group B after 24,48 and 72h ( P <0.05). Conclusion Both membrane meterial (polychloroethane) of heart assist pump and medical polyurethanes showed little cytotoxicity and good compatibility with cells. The former was better than the latter.更多还原