【摘要】 目的 :优化中药山楂中熊果酸的提取工艺。方法 :以熊果酸的含量和干浸膏得率为检测指标 ,采用L9(34)正交试验 ,考察溶剂浓度 (A)、溶剂用量 (B)、提取时间 (C)和提取次数 (D)四个影响因素 ,采用高效液相色谱法进行含量测定。结果 :因素A对提取物中熊果酸的含量有显著影响 ,其它因素影响不显著 ,最佳提取工艺条件为A3 B2 C2 D2 ,即 8倍量 95 %乙醇热回流 2次 ,每次 2h。结论 :优选得到的工艺稳定、方法可行。更多还原

【Abstract】 To optimize the extraction process of ursolic acid from Fructus Crataegi. Methods: The four relevant factors—solvent concentration (A), solvent volume (B), extraction time (C) and extraction times (D) were investigated by the L9(34) orthogonal design with the content of ursolic acid and the rate of the extracts as the index through content determination by HPLC. Results: The factor A is striking, and the other factors are not striking, and the optimum extraction condition is A3B2C2D2—8 fold of 95% ethanol, decocting for 2 times, each time for 2 hours. Conclusion: The optimized process is stable and efficient.更多还原