【摘要】 目的　为获得蜘蛛拖丝蛋白全基因。方法　以斑点杂交、Southern印迹结果证实了的蜘蛛拖丝蛋白全基因的克隆 ,构建其亚克隆文库 ,采用鸟枪法测序的策略 ,选取以HinfI为酶切工具 ,对Scos-DS1的DNA做大量酶切 ,以pUC1 9为载体 ,取插入片段大小为 5 0 0bp左右的阳性重组子 1 0 0个构建拖丝蛋白基因的亚克隆文库。结果　成功地构建了蛛丝蛋白基因Scos -DS1由HinfI酶切片段连接而成的亚克隆文库 ,得到了其部分序列。结论　亚克隆方法可以得到拖丝蛋白基因全序列更多还原

【Abstract】 Objective To obtain the whole length gene of dragline.Methods The Scos-DS1 DNA was digested with Hinf I and the subclone library of dragline was constructed using pUC19 as the vecter .After screening the library, 100 clones with insert around 500 bp in size were taken to determine the sequence.Results The subclone library of dragline gene connected by enzymolytic fragment of HinfI was succesfully constructed and its partial sequences were gained. Conclusion The whole length gene of dragline can be gained by using subclone method.更多还原