【摘要】 探讨局部应用扇贝多肽(PCF)对长期长波紫外线(UVA)辐射无毛小鼠皮肤所致突变型p53,表皮生长因子受体(EGFR)和P物质(SP)表达的影响。建立长期长波紫外线辐射无毛小鼠皮肤模型,免疫组化法测定皮肤组织突变型p53,表皮生长因子受体和P物质的表达。无毛小鼠背部皮肤每天一次应用5％和20％扇贝多肽可显著降低长期长波紫外线辐射(剂量为4556.4 J·cm-2)所致突变型p53,表皮生长因子受体和P物质的过表达。和模型组相比,5％扇贝多肽可分别降低突变型p53,表皮生长因子受体和P物质的表达至86.7％,81.7％和85.2％。20％扇贝多肽几乎可完全对抗长期长波紫外线辐射所致的过表达。扇贝多肽可通过抑制无毛小鼠皮肤中突变型p53,表皮生长因子受体和P物质的过表达,从而可保护皮肤防止光致癌和光老化。更多还原

【Abstract】 To investigate the effects of locally applied polypeptide from Chlamys farreri (PCF) on chronic ultraviolet A (UVA)-induced mutant type p53, epidermal growth factor receptor (EGFR) and substance P expressions in hairless mice skin. Chronic UVA damage model on hairless mice skin was established. Immunohistochemical analysis was employed to examine the expressions of mutant type p53, EGFR and substance P. Local application of PCF (5 % and 20 %) on murine dorsal skin once a day could significantly down-regulate the expressions of mutant type p53, EGFR and substance P in hairless mice skin irradiated by chronic UVA (total radiation intensity was 4556. 4 J·cm-2). 5 % PCF decreased the expressions of mutant type p53, EGFR and substance P to 86. 7 %, 81. 7 % and 85. 2 % of UVA model respectively, and 20 % PCF almost abolished these over expressions. PCF exerted strongly inhibitory effects on the overexpressions of mutant type p53, EGFR and substance P in hairless mice skin, and thus might protect skin from photocarcinogenesis and photoaging.更多还原