【摘要】 采用同一制膜方法分别对异常汉逊酵母、枯草芽孢杆菌和恶臭假单胞菌进行等量包埋制备固定化微生物膜,并采用自制的光化学BOD微生物传感器试验了各种膜的响应时间、线性范围、重现性等方面的性能,从而选择性能最佳的耗氧微生物。同时也考察了温度、pH、盐度对不同微生物膜的影响。实验结果表明:以恶臭假单胞菌膜制备的传感膜性能最佳,测定BOD时间低于20 min,方法的重现性及精密度较好,其荧光强度变化速率最大值(dI/dt)的相对标准偏差为2.2%,在BOD值0~80 mg·L-1范围内呈现良好的线性关系,相比之下更适用于BOD的快速检测。更多还原

【Abstract】 After immobilizing three kinds of limnetic microorganisms on sol-gel ormosils, three BOD sensors that used these microbial sensing films were investigated. For the BOD sensing films immobilized Hansennula anomala, Bacillus subtilis and Pseudomonas putida, the linear fluctuant coefficient (R^2) was in the range of 0-160 mg·L-1 and 0. 958 5 for Hansennula anomala, 0-160 mg·L-1 and 0. 956 0 for Bacillus subtilis , 0-80 mg·L-1 and 0. 990 6 for Pseudomonas putida, respectively. Reproducible responses could all be obtained below 3%. The effect of pH and sodium chloride concentration to the three microbial films were studied. After comparing these microbial films, sensing film with Pseudomonas putida is the best in respect of sensitivity, stability and reproducibility.更多还原