【摘要】 研究了提取介质、超声条件和洗脱条件对海洋微藻多糖提取纯化效果的影响。实验结果表明不同 p H的提取介质可以提取得到不同种类的多糖 ,超声条件以 3 0 0 W、 2 0 m in最佳 ,用葡聚糖凝胶 Sephadex-G2 0 0柱 (长 50cm,直径 2 cm)纯化粗多糖 ,当加样量为 4ml,用 0 .2 mol/L Na Cl溶液洗脱效果最好更多还原

【Abstract】 The paper investigates the effect of extracting medium, ultrasonic con dition and eluting condition on extraction and purification of polysaccharides f rom marine microalgae. The experimental results indicate that the resulting poly saccharides from marine microalgae are distinct as varied pH in medium. The opti mal ultrasonic power is 300W and the optimal ultrasonic duration is 20min. When the coarse polysaccharides are purified through the Sephadex-G200 column (lengt h: 50cm, diameter: 2cm),the optimal volume of sample added into the column is 4mL, and the optimal eluant is 0 2 mol/L NaCl.更多还原