【摘要】 目的　探讨丹那唑对大鼠实验性子宫内膜异位症模型c myc基因转录的影响及c myc基因在子宫内膜异位症发病中的作用。方法　根据Vernon采用自体子宫内膜盆腔移植法改进建立子宫内膜异位症动物模型 ,将建模成功大鼠随机分为非治疗组、丹那唑组 ,取正常鼠设为正常对照组。采用逆转录多聚酶链反应 (RT PCR)技术 ,半定量检测各组大鼠在位、异位子宫内膜c myc基因转录水平。结果　非治疗组在位内膜c myc基因转录水平高于正常组 (0 .2 9) ,而其异位内膜c myc基因转录水平也稍高于正常组 (0 .1 4 ) ;丹那唑组异位内膜相对在位内膜c myc基因转录水平显著高于非治疗组 (P <0 .0 5 )。结论　子宫内膜异位症在位、异位内膜c mycmRNA的表达较正常稍高 ;丹那唑通过促进异位内膜c myc基因转录 ,从而促进异位内膜组织的细胞凋亡 ,以达到其治疗目的更多还原

【Abstract】 Objective To investigate effects of Danazol on gene transcription in experimental rat models and its effects on pathogenesis of endometriosis.Methods Endometriosis model in rats was made using Vernons’method ,we established animal models using rats.Rats with successful models were randomized into untreated group and Danazol group.Normal rats were selected as the control group.Gene transcription of c myc in both eutopic and ectopic endometrium of endometriosis were investigated using semi quantitative RT PCR.Results Transcription of c myc mRNA in eutopic endometrium of untreated group was higher than that of control group(0.29).Transcription of c myc mRNA in ectopic endometrium of untreated group was higher than that of control(0.14).The expression of c myc mRNA in ectopic endometrium was higher than that in eutopic endometrium.There difference in Danazol group was significantly greater than those in untreated group.Conclusion Expression of c myc in ectopic endometrium of endometriosis was higher than that in normal endometrium.Through increasing transcription of c myc,Danazol might increase cellular apoptosis of ectopic endometrium to treat the disease.更多还原