【摘要】 目的　采用HPLC测定板蓝根提取物中靛蓝和靛玉红含量。方法　将板蓝根醇提液挥去乙醇 ,经氯仿萃取 ,然后用HPLC测定。色谱柱为DikmaDiamonsilTMC18(5 μm ,2 0 0mm× 4 6mm) ,流动相为甲醇 - 0 1%醋酸铵 -醋酸 (70∶30∶1) ,流速为1 0ml·min-1,检测波长 2 80nm。结果　靛蓝线性范围在 0 0 15 2～ 0 30 4 0 μg(r =0 9997) ,平均加样回收率为 97 3% ,RSD =1 87% (n =5 ) ;靛玉红线性范围在 0 0 16 5 6～ 0 3312 μg ,平均加样回收率为 96 6 % ,RSD =2 .32 % (n =5 )。结论　所用方法简便快捷 ,适合板蓝根药材以及含靛蓝、靛玉红产品的质量控制。更多还原

【Abstract】 OBJECTIVE To develop a method to determine indigo and indirubin contents in the alcohol extract of Radix isatidis by HPLC. METHODS Radix isatidis was extracted with ethanol, then extracted with trichloromethane.The chromatographic conditions were listed below: Diamonisil ODS column(5 μm,200 mm×4.6 mm), a mobile phase of methanol-0.1 mol·L -1 ammonium acetate-acetic acid(70∶ 30∶ 1),the flow rate of 1.0 ml·min -1 , The UV detection wavelength at 280 nm. RESULTS The calibration curves were linear in the range from 0.0152 μg to 0.304 μg(r=0.9997)for indigo and from 0.0166 μg to 0.3312 μg(r=0.9995)for indigo. The mean recoveries were 95.3%± 1.87% and 94.6±2.32% for indigo and indirubin, respectively. CONCLUSION The method was simple ,rapid and accurate to be used for quality control of alcohol extract of Radix isatidis and the product containing the indigo and indirabin.更多还原