【摘要】 目的 建立测定受试者口服乌拉地尔缓释胶囊后乌拉地尔血药浓度的方法。方法 采用RP-HPLC法,以1,3-二甲基-4-(γ-氯丙基氨基)-尿嘧啶为内标,乙腈-0.05 mol·1-1KH2PO4溶液-三乙胺-冰乙酸(20∶80∶0.1∶0.1)为流动相,检测波长270 nm。结果 血浆中乌拉地尔的平均回收率为98.9％±5.15％,日内RSD<7.37％,日间RSD<12.5％;在室温条件下乌拉地尔血浆样品至少能稳定8 h,在冰冻条件下至少能稳定30 d。结论 方法准确、简便、重复性好。更多还原

【Abstract】 OBJECTIVE To establish an RP - HPLC method for the determination of Urapidil in plasma from 24 healthy male volunteers who have been treated with Oral Urapidil sustained - release capsules. METHODS Samples were determined by RP - HPLC. The condition was acetonitril - 0.05 mol·l-1KH2PO4- triethylamine- glacial acetic acid(20:80:0.1:0.1) as the mobile phase and 270 nm as the detective wavelength. RESULTS The average recovery for Urapidil in plasma was 98.9%±5.15%. Within - day and between - day precision( RSD) were less than 13% .The plasma samples of Urapidil was stable within 8 hours at room temperature and within 30 days by freezing. CONCLUSION The method is accurate,simple and well reproducible.更多还原