【摘要】 目的　探讨钛颗粒对体外培养诱骨条件下的大鼠骨髓间充质干细胞增殖的影响。方法　采用四唑盐比色法测定诱骨条件下的大鼠骨髓间充质干细胞在钛颗粒负荷后的吸光度值 ,并用共聚焦激光扫描显微镜观察细胞形态变化。结果　钛颗粒负荷下 ,诱骨条件下的大鼠骨髓间充质干细胞的增殖能力受到抑制 ,不同直径 ,不同浓度和不同作用时间有程度各异的抑制作用。三种钛颗粒负荷中直径为 0 .9μm钛颗粒对诱骨条件下的大鼠骨髓间充质干细胞的抑制作用最显著 ;相同直径下 ,抑制程度也受浓度和时间影响 ,1g/L的抑制作用最强 ,出现抑制作用的时间最早 ,浓度越低 ,抑制作用越弱 ;钛颗粒负荷时间越长 ,增殖能力的抑制最显著。结论　钛颗粒负荷后诱骨条件下的大鼠骨髓间充质干细胞增殖能力受到抑制 ,且其抑制作用与钛颗粒的直径、浓度、时间有关。更多还原

【Abstract】 Objective To assess the effect of titanium particles on the proliferation of rMSCs after osteoblastic induction. Methods MTT-assay and Confocal Laser Scanning Microscopy are utilized to detect rMSCs’ proliferation rate and morphologic variety. Results It was found that Ti particles loading suppressed the proliferation of osteogenesis behavior of rMSCs, but the down-regulation effect varied in different circle diameter, different concentration and different loading duration of Ti particles. Especially, among three different particles, the submicron Ti particles (0.9 μm) had the most suppressive effect on rMSCs. Under the same diameter condition, the inhibitory effect induced by Ti particles loading showed a manner dependent on the particles concentration and exposure duration. The reductive response from 1 g/L Ti was the earliest and most intense one to come forth. And the lower the concentration was in use, the weaker would be the reductive response. Furthermore, with the elongation of exposure to Ti particles, proliferation was steadily decreasing and its lowest level was at 32 h of exposure. Conclusion These findings demonstrated that Ti particles loading could suppress the viability of rMSCs’ osteoblastic differentiation in a manner dependent on the circle diameter, particles concentration, and treatment time.更多还原