【摘要】 目的　观察体内青石棉表面的细微变化 ,为进一步研究石棉致病机制提供依据。方法　大鼠体内染尘后 ,用电子显微镜观察其在动物体内的变化 ,并用红外光谱、差热分析等谱学手段分析。结果　机体蛋白质在与青石棉的相互作用过程中 ,组织结构对称性降低 ;青石棉的硅已与蛋白质中的烷基、胺基交联成键 ,存在明显的 Si-O- C(N)、Si- R新的吸收谱带。机体以吞噬、包裹、缠绕 ,或以生化溶解方式排解粉尘 ,纤维自身则出现变短、尖部圆化、折断、分叉现象 ,也可以溶解、迁移、表面化学反应等。结论　石棉纤维表面基团和组织内的某些蛋白反应形成新的表面介体。肺泡内纤维的弱碳酸盐化现象是体内纤维溶解和反应的新形式。青石棉残留的粉尘仅为以 Si- O为主的物质。组织膜是体内粉尘迁移的隔离阻滞带。更多还原

【Abstract】 Objective To further study the pathogenic mechanism of crocidolite,the imperceptible changes of crocidolite surface in rat were observed. Methods The animal model was established and the changes in the rat infected with dust were observed by use of microscopy, SEM, differential thermal analysis and IR spectroscopy. Results In the course of interaction between organism protein and crocidolite, the protein symmetry decreased and structure loosened. The silicon of crocidolite was bonded with the alkyl, amido- of protein. New absorption bands of Si-O-C(N), Si-R clearly appeared. The organism cleared the dust by means of dissolution, enwrapping, winding or in the way of biochemical dissolution, and the fibre became shortened, broken, bifurcated, ends-rounded, and also it could dissolve, transfer and chemically react on surface. Conclusion The results showed that the surface radicals of asbestos fibre reacted with some albumen in tissue and hence formed new surface mediator. It is a new form of dissolution and reaction of fibre in vivo that fibres in alveoli transform to carbonate. The residual substances of crocidolite are mainly Si-O. Tissue membrane is the retardation cingulum of dust transference in vivo.更多还原