【摘要】 运用荧光光谱、傅立叶变换红外光谱以及曲线拟合等技术研究了镧离子对辣根过氧化物酶的结构的影响 .结果表明荧光增强的原因是由于镧离子的加入使镧离子和酶结合 ,酶的二级结构发生变化 ,导致荧光发色基团所处的微环境也相应变化 ;并运用它推测了镧离子对该酶的激活作用机制 .更多还原

【Abstract】 Through the fluorescence spectra, FT-IR spectra and curve-fit, the effect of lanthanum(III) on horseradish peroxidase (HRP) was studied. It is concluded that the reason of the stimulation is due to the binding of La(III) to HRP, which induced the changes of the enzyme’s secondary structure and the microenvironment of the fluorophore, while adding La(III) into the HRP. The activation mechanism of La(III) to the HRP was also discussed.更多还原