【摘要】 目的　探讨p16和nm2 3基因在唾液腺良、恶性肿瘤中的表达情况及与唾液腺肿瘤发生之间的关系。方法　用免疫组织化学SABC法检测 39例正常唾液腺和唾液腺肿瘤存档石蜡标本中P16蛋白和nm2 3蛋白的表达。结果　P16和nm2 3蛋白阳性表达主要见于细胞浆及细胞核 ,呈棕黄色。在正常唾液腺中 ,二者阳性表达率均为 10 0 %(4 4 )。在良性和恶性唾液腺肿瘤中 ,P16蛋白阳性表达率分别为 76 9% (10 13)和 4 0 9% (9 2 2 ) ,良恶性之间P16蛋白表达差异有统计学意义 (P <0 0 5 ) ;nm2 3蛋白阳性率分别为 84 6 % (11 13)和 4 5 5 % (10 2 2 ) ,良恶性之间亦有统计学意义 (P <0 0 5 )。唾液腺肿瘤中P16与nm2 3表达之间无相关性。结论　p16和nm2 3基因在唾液腺肿瘤发生中可能分别起着不同的重要作用 ,其表达下降可能有利于唾液腺恶性肿瘤的形成。更多还原

【Abstract】 Objective To study the expression of p16 and nm23 genes in salivary gland tumors and the relation of P16 and nm23 proteins with tumorigenesis of salivary gland tumors.Methods Expression of P16 and nm23 proteins was examined by SABC immunohistochemical method in 39 cases of paraffin blocks of normal salivary gland tissues and salivary gland tumors.Results P16 and nm23 protein positive staining were mainly found in the cytoplasm and cytoblast of all salivary gland tissues. Positive rate of P16 protein expression was 76.9%(10/13) and 40.9%(9/22) in benign and malignant salivary gland tumors, respectively. There was signioficant difference between P16 protein expression of benign and malignant tumors by χ+2 test (P<0.05). nm23 protein positive staining was found in 84.6%(11/13) and 45.5%(10/22) of benign and malignant tumors respectively. The expression of nm23 protein in benign and malignant tumors was significantly different (P<0.05). There was no correlation of the expression of P16 and nm23 in salivary gland tumors was found (P>0.05).Conclusion p16 and nm23 genes may play an important role in different sides in salivary gland tumorigenesis and the reduce of the expression of p16 and nm23 genes may contribute to the generation of malignant salivary gland tumors.更多还原