【摘要】 目的　探讨骨骼肌失神经的退变和再生。方法　以纯系BALB C小白鼠为实验动物 ,切断一侧坐骨神经 ,术后 1,2 ,4 ,8,12 ,16周分别处死动物 ,取腓肠肌分别进行光电镜观察和免疫组化研究。结果　骨骼肌失神经早期主要为肌纤维萎缩 ,随后退变和再生 ,较长时间失神经后再生的肌细胞再发生退变。失神经后 1～ 4周肌动蛋白和肌红蛋白表达均降低 ,8周出现较小的细胞阳性染色 ,肌纤维阳性染色主要位于核周 ,12～ 16周大片的细胞核周有较浅的阳性胞浆染色。这些细胞不具正常肌纤维形态。结论　骨骼肌失神经后退变和再生同时存在 ,由于无神经支配 ,再生的肌细胞不能分化发育为成熟的肌纤维 ,而再发生退变 ;较长时间失神经后再生的成肌细胞相互融合呈片状分布 ,未能发育为成熟的肌纤维。更多还原

【Abstract】 Objective To study the degeneration and regeneration of skeletal muscle after denervation.Methods Denervation was carried out in gastrocnemius muscles in 30 adult BALB/C mice by cutting the sciatic nerve. The gastrocnemius muscles were removed at 1,2,4,8,12,16 weeks after denervation, respectively. Specimens were processed for histological study and immunohistochemical technique.Results Muscle fiber atrophy followed by degerneration and regeneration was observed in the early period of denervation. Fusion of the regenerated muscle cells with each other followed by degeneration of the cells and growth of fibroconnective tissue were observed in the later stage. The expression of myoglobin and actin decreased in 1～4 weeks after denervation. The postive expression of the proteins was observed in some 8 weeks′ cells and in many degenerated 12～14 weeks′ muscle cells.Conclusion Degeneration and regeneration may coexisted in the denervated muscles. The regenerated muscle cells can′t fully develop due to the deficit of nerve regulation and degenerate again. The regenerated muscle cells will melt each other and can′t develop to mature muscle fiber in the later stage.更多还原